Analysis Note

ControlRat brain lysate.

Application

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Anti-Vesicular GABA Transporter Antibody is an antibody against Vesicular GABA Transporter for use in WB, IH.

Immunohistochemistry Analysis:
1:50 - 1:500 dilution from a previous lot detected vesiluar GABA transporter using IHC-Select Detection with HRP-DAB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Vesicular GABA transporter (VGAT), the first vesicular amino acid transporter to be molecularly identified, differs in structure from previously cloned vesicular neurotransmitter transporters and defines a novel gene family. VGAT is highly concentrated in the nerve endings of GABAergic neurons in the brain and spinal cord but also in glycinergic nerve endings. In contrast, hippocampal mossy fiber boutons, which are glutamatergic but known to contain GABA, lack VGAT immunoreactivity. Although the great majority of nerve terminals containing GABA or glycine are immunopositive for VGAT, subpopulations of nerve endings rich in GABA or glycine appear to lack the protein. Additional vesicular transporters or alternative modes of release may therefore contribute to the inhibitory neurotransmission mediated by these two amino acids.

Immunogen

KLH conjugated Vesicular GABA Transporter corresponding to the C-terminus.

Epitope: C-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Rabbit polyclonal serum in buffer containing 0.1M Tris-Glycine (pH7.4) 15mM NaCl with 0.05% NaN3.

Antigen Affinity Purified

Quality

Routinely evaluated by Western Blot on rat brain lysates.

Western Blot Analysis:
1:500 - 1:1,000 dilution of this lot detected vesicular GABA transporter on 10 µg of rat brain lysate.

Specificity

Cat. # AB2257 recognizes VGAT.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

Approx. 57 kDa