Analysis Note

ControlPurkinje cells in rat mid-brain region

Application

Anti-GABA B Receptor R2 Antibody is an antibody against GABA B Receptor R2 for use in IH & WB.

General description

Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA exerts its effects through ionotropic [GABA(A/C)] receptors, to produce fast synaptic inhibition, and metabotropic [GABA(B)] receptors, to produce slow, prolonged inhibitory signals. The GABA(B) receptor consists of a heterodimer of two related 7-transmembrane receptors, GABA(B) receptor 1 and GABA(B) receptor 2. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates 4 transcript variants.

Immunogen

Bovine thyroglobulin-conjugated linear peptide at the C-terminus of rat GABA-B-R2.

Linkage

Replaces: AB5394

Quality

Evaluated by Immunohistochemistry in Purkinje cells in rat mid-brain region.

Immunohistochemistry Analysis: A 1:300 dilution of this antibody detected GABA-B-R2 in Purkinje cells in rat mid-brain region.

Specificity

This antibody recognizes the C-terminus of rat GABA-B-R2.

Target description

Although a band is observed around the calculated molecular weight of 105 kDa, Western Blotting is not a recommened application for this antibody because the band is weak in comparison to other non-specific bands present.