LAMININ; RBX-100UG

Application

IFA (with fluorescein anti-rabbit IgG conjugate) - use diluted at ≥ 1:80 on frozen mouse tissues (skin, liver, etc.).
IHC (paraffin) - use diluted at ≥ 1:500 on fixed, paraffin-embedded mouse tissues (skin, liver, etc). (immunoperoxidase technique with the Envision / Dako kit);
ELISA - use diluted at ≥ 1:200 (OD ≥ 500).
Optimal working dilutions must be determined by the end user.

Research CategoryCell Structure

Research Sub CategoryECM Proteins

Anti-Laminin Antibody detects level of Laminin & has been published & validated for use in ELISA, RIA, IH.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Laminin extracted and purified from EHC mouse tumor tissues.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

The purified antibody is supplied in a buffer containing a mixture of 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10µg/ml of antibiotics and antimytotics

Specificity

Laminin extracted and purified from mouse tumor tissues. Antibody shows less than 0.1% reactivity with mouse collagen Types I, II, and IV; rat collagen Types I, III, and V; rat and human elastin; mouse fibronectin and laminin by radioimmunoassay.

Storage and Stability

Maintain frozen at -20°C for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.