Application

Western blot: 1:2,000

Immunoprecipitation: 1:500

MMP-2 [Gelatinase-A] is constitutively produced in quiescent cells and tissues, and the enzyme has a high specific activity against denatured collagen. The low protein levels produced in cell culture (pg/mL) are often below the threshold of detection by standard Western Blotting. The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, 1992) or immunoprecipitation. This can prevent appearance of a spurious band of antibody binding in the vicinity of 68 kDa displayed by some concentrated media.

Optimal working dilutions must be determined by end user.

Research CategoryCell Structure

Detect MMP-2 using this Anti-MMP-2 Antibody, catalytic domain validated for use in IP & WB.

Research Sub CategoryMMPs & TIMPs

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: catalytic domain

E. coli-expressed active rat 72 kDa type IV collagenase (catalytic domain)

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1048

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin. Liquid in PBS, containing 0.05% sodium azide.

Format: Purified

Specificity

Recognizes rat MMP-2. Exhibits no cross-reactivity with other MMP family members. The antibody was generated using E. coli-expressed active rat 72 kDa type IV collagenase (catalytic domain) as an immunogen.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.