Application
Western blot: 0.5 - 1.0 µg/mL; recognizes 250-350 kDa polypeptides in reduced samples.
Immunohistochemistry: 10 to 20 µg/mL in paraffin embedded tissues
Optimal working dilutions must be determined by end user.
This Anti-Tenascin Antibody is validated for use in WB, IH(P) for the detection of Tenascin.
Research CategoryCell Structure
Research Sub CategoryECM Proteins
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Tenascin is known to play an active role in development of central nervous system and mesenchymal-derived organs, exist in the vasculature of tumors in adults, and function in cell adhesion.
Immunogen
Purified human tenascin from glioma culture supernatant.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form
Affinity purified using immobilized human tenascin; presented as a liquid in PBS containing no preservatives.
Specificity
Reacts strongly with tenascin from human and mouse in both Western blotting and immunohistochemistry. While it has not been tested, it should also react strongly in these applications with tenascin from all mammalian species.
Reactivity with other species has not been confirmed, but likely to react with all mammalian species.
Storage and Stability
Maintain at -20°C for up to 12 months from date of receipt. Aliquot to avoid repeated freeze/thaw cycles.
This product has met the following criteria to qualify for the following awards: