Application

ELISA: 0.5 µg/mL this dilution will allow detection (in direct ELISA) of 0.2-0.4 ng/well when human SDF-1alpha is used to coat the plate.

Western blot: 0.1-0.2 µg/mL, this dilution should offer a detection limit of 1.5-3.0 ng/lane, under reducing or non-reducing conditions.

Optimal working dilutions must be determined by the end user.

Neutralization: To yield one-half maximal inhibition (ND50) of the biological activity of human SDF-1alpha (100 ng/mL), a concentration of 2.0-0.4 ng/well of recombinant human SDF-1alpha

Research Sub CategoryCytokines & Cytokine Receptors

Research CategoryInflammation & Immunology

Detect Stromal Cell-Derived Factor-1α using this Anti-Stromal Cell-Derived Factor-1α Antibody validated for use in ELISA, WB, NEUT.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Highly pure (>98%) recombinant human SDF-1alpha.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Affinity purified immunoglobulin. Lyophilized from 0.5X PBS pH 7.2. Reconstitute with sterile distilled water.

Specificity

Reacts with human SCDF-1alpha

Storage and Stability

The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile water to a concentration of =0.2 mg/ml the antibody is stable for at least six weeks at 2-4°C.