Application

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Anti-Vesicular Monoamine Transporter 1 Antibody detects level of Vesicular Monoamine Transporter 1 & has been published & validated for use in ELISA, IH & WB.

Western blot: 1-10 µg/mL (Chemiluminescence technique)

Immunohistochemistry: 1-20 µg/mL on paraformaldehyde fixed sections.

ELISA: 0.5-1.0 µg/mL (1 µg/mL VMAT1 peptide (Catalog Number AG250)/well)

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

A 12 amino acid C-terminal peptide sequence from rat VMAT1 (Liu et al. 1992). This peptide is predicted to be cytoplasmic.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA.

Specificity

Vesicular Monoamine Transporter 1 (VMAT1). An antibody made to the C-terminal VMAT1 peptide detected a major band at ~55 kDa in postnuclear supernatants of CHO transfected with VMAT1 and not in wild type cells (Liu et al. 1994). Some additional bands, both higher and lower molecular weight, were also detected and remain unaffected by the inclusion of protease inhibitors. VMAT1 has been localized in adrenal chromaffin cells, endocrine and paracrine cells associated with the intestine, stomach and sympathetic nervous system (Liu et al. 1994; Weihe et al. 1994). VMAT1 has also been found in endosomes of transfected CHO cells and in LDCVs of rat PC12 cells (Liu et al. 1994). This antibody is not expected to cross-react with human VMAT1 (Erickson et al. 1996).

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.