Application

Detect Opioid Receptor using this rabbit polyclonal antibody, Anti-Mu Opioid Receptor Antibody validated for use in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:1,000-4,000 dilution from a representative lot detected Mu Opioid Receptor in human cerebellum, mouse hippocampus, mouse midbrain, and rat midbrain tissue.

General description

Mu Opioid Receptor (MOP), also called Mu-type opioid receptor (MOR-1), is a receptor for natural and synthetic opioids such as beta-endorphin, endomorphin, morphine, heroin, DAMGO, fentanyl, etorphine, buprenorphin and methadone. MOP functions as a class A G-protein coupled receptor (GPCR), but its down-regulation pathways vary with the agonist and may or may not be independent of G-protein coupling. Endogenous ligands induce rapid desensitization, endocytosis and recycling, as opposed to synthetic ligands, like morphine, that trigger only low desensitization and endocytosis. Hetero-oligomerization with other GPCRs can modulate agonist binding, signaling and trafficking properties. MOP is expressed in the brain and is involved in neurogenesis. MOP is the main physiological target for most clinically important opioid analgesics.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human Mu Opioid Receptor.

Linkage

Replaces: AB1580

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Mu Opioid Receptor in 10 µg of SH-SY5Y cell lysate.

Specificity

This antibody recognizes the C-terminus of Mu Opioid Receptor. This antibody will recognize isoform 1 (45 kDa), isform 10 (55 kDa), isoform 12 (34 kDa), and isoform 13 (36 kDa).

Target description

~70 kDa observed. The calculated molecular weight is 45 kDa, however Opioid Receptor mu has been shown as a ~70-90 kDa band in western blots (Ferrer-Alcon, M., et al. (2004). Molecular Brain Research. 121(1-2):114-122).