Anti-Beta III Tubulin; Cy3 Conjugate

Analysis Note

ControlRat E18 cortex cells

Application

Research CategoryNeuroscience

Research Sub CategoryNeuronal & Glial MarkersNeurofilament & Neuron Metabolism

This Anti-Beta III Tubulin, Cy3 Conjugate Antibody is validated for use in ICC for the detection of Beta III Tubulin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tubulin is one of several members of a small family of globular proteins which polymerize to form cytoskeletal structures called microtubules. The most common members of the tubulin family are α-tubulin and β-tubulin. Tubulin dimers, typically composed of α- and β-tubulin subunits, assemble onto microtubules forming large polymers. β-III tubulin is a unique microtubule subunit that is found expressed almost exclusively in neurons. Due to the unique expression pattern of β-III tubulin it is often utilized as a marker to identify neuronal cells from other brain cells such as glia which do not express it. β-III tubulin also serves as a cytoarchitectural marker since it is primarily found within neurite extensions.

Immunogen

Synthetic linear peptide corresponding to human Beta III Tubulin.

Physical form

Affinity purified

Purified rabbit polyclonal conjugated to Cy3 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Quality

Evaluated by Immunocytochemistry in rat E18 cortex cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Beta III Tubulin in rat E18 cortex cells.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

50 kDa calculated