NGF; SH X-500UG

Application

Immunohistochemistry: 1-10 µg/mL (see suggested protocol).

Immunoblotting: 1-10 µg/mL

ELISA: 1-10 µg/mL

Inhibition of biological activity in vitro: 1-10 µg/mL
BIOLOGICAL ACTIVITY: Neutralizes NGF, but not other neurotrophins.

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

Research Sub CategoryNeurochemistry & Neurotrophins

Anti-Nerve Growth Factor-β Antibody is an antibody against Nerve Growth Factor-β for use in ELISA, WB, IH.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Mouse nerve growth factor-beta purified from salivary glands. Antigen purity greater than 95% by PAGE.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1119

Physical form

Protein G purified immunoglobulin. Lyophilized from PBS, no preservatives. Reconstitute with 500 µL of sterile distilled water (1 mg/mL).

Format: Purified

Specificity

Nerve growth factor-beta (NGF). By one site ELISA, less than 1% cross-reactivity against recombinant human BDNF or neurotrophin 4. Approximately 2% cross-reactivity against neurotrophin 3.

Storage and Stability

Maintain at -20 to -70°C in undiluted aliquots for up to one year. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.