Application

Research CategoryNeuroscience

Research Sub CategoryNeurochemistry & Neurotrophins

Anti-Nerve Growth Factor-β Antibody detects level of Nerve Growth Factor-β & has been published & validated for use in ELISA, WB, IH.

Immunohistochemistry: 1-2 µg/mL (see suggested protocol).

Immunoblotting: 1-2 µg/mL

Inhibition of biological activity: 1-2 µg/mL
BIOLOGICAL ACTIVITY: This antibody will completely inhibit the survival and neurite outgrowth actions of mouse NGF in chicken dorsal root ganglion neurons in vitro.

ELISA: 1-2 µg/mL

Use neat for in vivo animal studies.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Mouse nerve growth factor-beta purified from salivary glands.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1119

Physical form

Purified immunoglobulin. Lyophilized from PBS. Contains no preservative. Reconstitute with 500 µL of sterile distilled water (1 mg/mL).

Format: Purified

Specificity

Nerve growth factor-beta (NGF). Less than 1% cross-reactivity with recombinant human NT3, NT4 and BDNF by ELISA.

Storage and Stability

Maintain at -20 to -70°C in undiluted aliquots for up to one year after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) may be added for additional stability. Avoid repeated freeze/thaw cycles.