Analysis Note

ControlThe control peptide is available for absorbtion studies. Please see catalog number AG617.

Application

Anti-Glucose Transporter GLUT-3 Antibody, C-terminus is an antibody against Glucose Transporter GLUT-3 for use in ELISA & WB.

Western blotting: 1:1,000-1:5,000, using chemiluminescent detection. Detects a 45-48kDa band in membrane preps from rat brain. Slight differences in band sizes in different tissues; reported range is 45-55kDa.

Other detection methods may require a higher concentration of antibody.

ELISA: 1:10,000 - 1:50,000, using 50-100 ng of antigen/well.

Optimal working dilutions must be determined by end user.

Immunogen

Synthetic peptide corresponding to the C-terminus of mouse Glut-3 coupled to KLH. Product sold sold as AG617

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Liquid rabbit antiserum containing 0.05% sodium azide.

Specificity

Recognizes Glut-3 in mouse and rat tissues. C terminal peptide used shows no homology to the human sequence.

Storage and Stability

Store at -20°C for up to 12 months after date of receipt in undiluted aliquots. Avoid repeated freeze-thaw cycles.