Analysis Note

ControlMouse kidney lysate

Application

Anti-Angiopoietin-1 Antibody is an antibody against Angiopoietin-1 for use in WB.

General description

The remodelling of the primitive vasculature in mammals to form the mature cardiovascular system includes a number of factors directing endothelial cell development. Angiopoietin-1 is an angiogenic secreted protein that interacts with the endothelial specific Tie-2 receptor. It is primarily expressed in developing endothelial cells. During embryonic development, Ang-1 binds and induces tyrosine phosphorylation of Tie-2. Ang-1 appears to play a crucial role in mediating matrix and mesenchyme. It mediates blood vessel maturation and stability. Ang-1 may play a critical role in heart development. Ang-1 deficient mice mimic the phenotype exhibited by animals deficient in Tie-2. A homolog of Ang-1, termed Angiopoietin-2 may act as an antagonist for Ang-1 and Tie-2. Ang-1 and Ang-2 have ~60% sequence homology.

Immunogen

This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from mouse Angiopoietin-1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Quality

Evaluated by Western Blot in mouse kidney lysate.
Western Blot Analysis: : 1:1,000 dilution of this lot detected Angiopoietin-1 on 10 µg of mouse kidney lysate.

Specificity

This antibody recognizes Angiopoietin-1.

Target description

57 kDa