Analysis Note

ControlImmunohistochemistry Positive control: surrounding cartilageWestern blotting positive control: total 13 day mouse embryo

Application

Anti-Versican Antibody, a.a. 1360-1439 of mouse versican is an antibody against Versican for use in IP, WB, IH(P).

Immunohistochemistry (formally fixed, paraffin embedded): 10 µg/mL. Tissues must be treated with Chondroitinase before staining. {0.5U/mL in 100mM Tris-HCL pH 7.2-7.4, 30 minutes at room temperature} Then block and stain.

Western blotting: 0.5 µg/mL

Immunoprecipitation:

Optimal working dilutions must be determined by the end user.

General description

Versican is a large multi-domain chondroitin sulphate proteoglycan, is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. In addition, versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan., Versican has an apparent molecular mass of ca. 1000 kDa, of which the core contributes 400 kDa from the 2409 amino acids, and the 12 -15 covalently attached chondroitin sulphate chains make up the rest.

Immunogen

GST fusion protein containing amino acids 1360-1439 of mouse versican.

Epitope: a.a. 1360-1439 of mouse versican

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

The immunoglobulin was affinity purified against the recombinant antigen and is presented in PBS. Anti-GST reactivity has not been adsorbed. Liquid in PBS.

Format: Purified

Specificity

Mouse versican GAG beta domain.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeat freeze/thaw cycles.