Rabbit anti-Mouse BID Cleavage site spec

Analysis Note

ControlMouse L929 (3T3-L1) cells treated with TNF-α to induce cleavage; caspase-8 cleaved recombinant mouse BID.

Application

This Anti-mouse BID cleavage (59/60) site specific Antibody is validated for use in WB for the detection of mouse BID cleavage (59/60) site specific.

Research CategoryApoptosis & Cancer

Research Sub CategoryBCL2 & Inhibition

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

BH3 interacting domain death agonist (BID, 22 kDa) is a pro-apoptotic member of the Bcl-2 family. BID interacts with both Bcl-2 and Bax through its BH3 domain. It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase-8 in response to TNF-α or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase-8 activation and mitochondrial death machinery.

Immunogen

The antiserum was produced against a chemically synthesized peptide derived from the 15 kDa caspase-8 cleavage product of mouse BID.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Format: Purified

Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier, 0.05% sodium azide.

Specificity

Mouse 15 kDa caspase-8 cleavage product of BID. Human (75% conserved) BID has been tested and shown not to cross-react. Rat (100% conserved) and chicken (62% conserved) BID have not been tested.

Storage and Stability

Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.