Application

Avian proteins over expressed and purified from E. coli were analyzed by western blot using HRP-conjugated rabbit anti-chicken IgY as the secondary at 1:2000 dilution.

Anti-Chicken IgY (IgG) (whole molecule) FITC antibody has been used in immunohistology.

Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody has been used for ELISA. The antibody can also be used for dot blot (1:160,000) and IHC (1:500) assays.

Biochem/physiol Actions

IgY plays a major role in defense mechanism against systemic infections. It also possesses the ability to initiate anaphylactic reactions.

Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus anti-chicken antibodies are useful analytical tools for various research applications.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Chicken IgY is made of two heavy (H) and two light (L) chains. Its molecular weight is about ~180 kDa.

Immunogen

purified chicken IgY (IgG)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Specificity

Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody binds to chicken IgY.