Application

Anti-c-Myc Agarose Affinity Gel antibody produced in rabbit has been used in:chromatin immunoprecipitation (ChIP) co-immunoprecipitation western blottingimmunofluorescenceimmunoprecipitation

Biochem/physiol Actions

c-Myc proto-oncogene increased expression is observed in a variety of human tumors. Amplification of Myc gene locus is observed in angiosarcomas and translocation is implicated in Burkitt′s lymphoma. c-Myc is a master regulator and targets a wide variety of metabolic events. An epitope located within amino acid residues 410-419 (EQKLISEEDL) of human c-Myc has been widely used as a tag in many expression vectors, enabling the expression of proteins as c-Myc tagged fusion proteins. Anti-c-Myc agarose is useful in the purification of expressed c-Myc tagged fusion proteins in bacterial lysates or transfected cells.

Disclaimer

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General description

The human c-Myc proto-oncogene belongs to MYC oncogene family and is the human cellular homolog of the avian v-Myc gene found in several leukemogenic retroviruses. c-Myc gene is localized on human chromosome 8q24.21. The two major domains of include the N-terminal box regions and the C-terminal basic-region with helix-loop-helix/leucine-zipper motif.

Immunogen

synthetic peptide of the human p62^c-Myc protein.

Physical form

Suspension of beaded agarose (1:1 v/v) in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Specificity

Anti-c-Myc recognizes an epitope located on c-Myc tagged fusion proteins.

Storage and Stability

For continuous use and extended storage, store at 2 °C to 8 °C. Do not freeze.