ANTI-FLAG M2 Affinity Gel, purified immunoglobulin, buffered aqueous glycerol solution

Code: A2220-2X25ML D2-231

Application

Anti-FLAG® M2 affinity gel has been used for western blotting, immunoprecipitation and for the purification of FLAG fusion proteins.Browse additio...


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Application

Anti-FLAG® M2 affinity gel has been used for western blotting, immunoprecipitation and for the purification of FLAG fusion proteins.Browse additional application references in our FLAG® Literature portal.

General description

Anti-FLAG M2 Affinity gel is a mouse monoclonal antibody that is covalently attached to agarose. The antibody binds FLAG at the N-terminal, Met-N-terminal, C-terminal and internal locations of fusion proteins. Binding is calcium-independent.Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide

Immunogen

DYKDDDDK

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Physical form

Suspension in buffered saline containing azide as preservative and 50% glycerol

analyte chemical class(es)proteins (FLAG® tag, 3x FLAG®, DYKDDDDK tag)
antibody formpurified immunoglobulin
antibody product typeprimary antibodies
capacity>0.6 mg/mL, resin binding capacity (FLAG-BAP)
cloneM2, monoclonal
conjugateagarose conjugate
formbuffered aqueous glycerol solution
isotypeIgG1
matrix(4% agarose bead; 45-165µm bead size)
Quality Level200
shipped inwet ice
storage temp.−20°C
technique(s)affinity chromatography: suitable (FLAG® peptide, Glycine, pH3.5, 3x FLAG® peptide), immunoprecipitation (IP): suitable
This product has met the following criteria to qualify for the following awards:



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