Application

Anti-mouse IgG (Fc specific)–alkaline phosphatase antibody can be used in ELISPOT (immunospot) assay. It can also be used in immunohistochemistry and western blot.

Alkaline phosphatase-conjugated goat anti-mouse Fc specific antibody was used as a secondary antibody in ELISA assays at a dilution of 1:1000 in PBS/0.1% Tween and 1% BSA for 1.5 hours at 37°C. Antibody was developed using 4-nitrophenyl phosphate (Sigma) as a substrate for 30 minutes at 37°C.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. Anti-mouse IgG (Fc specific)–alkaline phosphatase antibody (diluted 1: 10,000 in PBS-Tween) can be used in ELISA. It is also useful in immunoblotting. Goat anti-mouse IgG (Fc specific)–alkaline phosphatase antibody reacts specifically with mouse IgG Fc fragment, IgG and its subclasses IgG1, IgG2a, IgG2b and IgG3 but not with human IgG, and mouse IgA or IgM.

Immunogen

Purified mouse IgG Fc fragment.

Other Notes

Antibody adsorbed with human IgG.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ and 15 mM sodium azide

Preparation Note

Adsorbed to reduce background with human samples.