Anti-Tie2 Mouse mAb (Ab33)

Application

Frozen Sections (see comments)r>Immunoblotting (0.2-1 µg/ml, chemiluminescence)r>Immunoprecipitation (see comments)r>Paraffin Sections (not recommended)

General description

Protein G purified mouse monoclonal antibody. Recognizes the ~140-145 kDa Tie2 protein.

Recognizes the ~140-45 kDa Tie2 protein.

This Anti-Tie2 Mouse mAb (Ab33) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Tie2.

Immunogen

a recombinant protein consisting of amino acids 1-745 of human Tie2 fused to a His Tag™ sequence

Human

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For frozen sections, tissues were treated with Triton^™-X-100 detergent. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Lin, P., et al. 1998. Proc. Natl. Acad. Sci. USA95, 8829.Wong, A.L., et al. 1997. Circ. Res.81, 567.Peters, K.G., et al. 1998. Br. J. Cancer77, 51.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)