Application

Sphingolipid ceramide N-Deacylase, Pseudomonas sp. has been used as a PP2A enhancer to study its effects on phosphatase and tensin homolog (PTEN) in forkhead box O3 (FOXO3a) phosphorylation in primary CD4⁺ T cells.

General description

Sphingolipid ceramide N-deacylase (SCDase) is a native sphingolipid ceramide N-deacylase fromPseudomonasspecies. It hydrolyzes the N-acyl linkage between fatty acids and the sphingosine base of ceramide in several sphingolipids and gangliosides. This enzyme catalyzes the condensation reaction between fatty acids and sphingosine bases and forms sphingolipids. It is also involved in transacylation reactions. SCDase is not recommended for hydrolysis of ceramide. It is also used to prepare lysoglycosphingolipids (lysoGSLs) effortlessly.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Mitsutake, S., et al. 1997. Anal. Biochem. 247, 52.Sueyoshi, N., et al. 1997. J. Lipid Res. 38, 1923.Ito, M., et al. 1995. J. Biol. Chem. 270, 24370.

Packaging

250 miu in Plastic ampoule

Physical form

In 50 mM sodium acetate buffer, 0.1% LUBROL™ PX Detergent, pH 6.0.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of asialo-GM₁ per min at 37°C, pH 6.0.

Warning

Toxicity: Standard Handling (A)