PKC#, Human, Recombinant, S. 1PC X 5UG

General description

Full-length, recombinant, human PKCδ expressed in S. frugiperda insect cells using a baculovirus expression system. PKC δ is classified as a novel PKC isozyme because it does not exhibit calcium dependence and it is activated in vitro by phorbol esters. Suitable for studies of PKCδ and its regulation. PKCδ is a Ca²⁺-independent and phospholipid-dependent enzyme.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hug, J. and Sarre, T.F. 1993. Biochem. J. 291, 329.Kazanietz, M.G., et al. 1993. Mol.Pharmacol. 44, 298.Koide, H., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 1149.

Packaging

5 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 250 mM NaCl, 20 mM HEPES, 5 mM DTT, 2 mM EDTA, 2 mM EGTA, 50% glycerol, 0.05% TRITON^™ X-100 Detergent, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PKC_ε Peptide Substrate (catNO=539562">Cat. No. 539562) per min at 30°C, pH 7.5.

Warning

Toxicity: Standard Handling (A)