PKG, I#, Bovine Lung 1PC X 4UG

General description

Native PKGIA purified from bovine lung. PKG is involved in regulation of smooth muscle relaxation, platelet function, and cell division. PKG is activated by binding of cGMP to regulatory subunits within the holoenzyme, but in contrast to PKA, the activation of PKG does not invlove physical dissociation of catalytic from the regulatory domains. The activated enzyme phosphorylates target proteins at serine and/or threonine residues that are found within the recognition motif, Arg-Lys-Arg-Ser*-Arg-Ala-Glu.

Note: 4µg = 6000 units.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Brophy, M.C., et al. 2006. J. Vasc. Res.39, 95.Colbran J.L., et al. 1992. J. Biol. Chem.267, 9589.Corbin, J.D. and Doskeland, S.O. 1983. J Biol Chem258, 11391.Witt, J.J. and Roskoski, R., Jr, 1975. Anal Biochem66, 253.

Packaging

4 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 25 mM β-mercaptoethanol, 10 mM K₂HPO₄, 1 mM EDTA, 12% sucrose, pH 6.8.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme required to incorporote 1 pmol phosphate into the synthetic heptapeptide substrate, RKRSRAE, in 1 min at 30°C.

Warning

Toxicity: Standard Handling (A)