Application

Immunoblotting (10 µg/ml)r>Neutralization Studies (see application references)

General description

Recognizes the ~50 kDa PAI-1 protein.

This Anti-PAI-1 Mouse mAb (MA-33B8) is validated for use in Immunoblotting, Neutralization Studies for the detection of PAI-1.

Purified mouse monoclonal antibody. Recognizes the ~50 kDa PAI-1 protein.

Immunogen

Human

human tPA-PAI-1 complex

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Rapidly inactivates PAI-1 (Plasminogen Activator Inhibitor-1) to the latent form. Like the native latent form, PAI-1 inactivated by antibody was protected from cleavage by elastase. Also shown to reduce renal fibrin deposits in a rabbit model of endotoxemia. Variables associated with assay conditions will dictate the proper working dilution.

Montes, R., et al. 2000. Thromb. Haemost.84, 65.Verhamme, I., et al. 1999. J. Biol. Chem.274, 17511.Debrock, S., and Declerck, P.J. 1997. Biochim. Biophys. Acta1337, 257.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM NaCl, 50 mM sodium phosphate buffer, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)