General description

Full-length, recombinant, human phosphodiesterdase 3A (PDE3A) expressed in S. frugiperda insect cells using a baculovirus expression system. PDE3A is expressed primarily in cardiac and vascular myocytes and platelets. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Shakur, Y., et al. 2000. J. Biol. Chem.275, 38749.

Packaging

10 u in Plastic ampoule

Physical form

In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that is required to convert 1 pmol cAMP to AMP per min at pH 7.5, 30°C.

Warning

Toxicity: Standard Handling (A)