General description

Full-length, recombinant, human phosphodiesterase 3B (accession no. AY459346) fused at the N-terminus to a His Tag™ sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. An important component of insulin and cAMP-dependent signaling. Supplied as a partially purified preparation.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39.Kitamura, T., et al. 1999. Mol. Cell. Biol.19, 6286.Degerman, E., et al. 1997. J. Biol. Chem.272, 6823.

Packaging

10 u in Plastic ampoule

Physical form

In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole cAMP to AMP per min at pH 7.5, 30°C.

Warning

Toxicity: Standard Handling (A)