General description

Recombinant, human MMP-13. MMP-13 is a collagenase-3 enzyme that degrades a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X, and XIV, gelatin, aggrecan, perlecan and fibronectin. It is distinguished from other human collagenases by the fact that it effectively degrades type II collagen. The importance of MMP-13 is further strengthened by reports that it is a biomarker for poor prognosis in many carcinomas and other type of cancers.

Legal Information

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Freije, M.P.S., et al. 1994. J. Biol. Chem.269,16766.

Packaging

5 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM HEPES, 10 mM CaCl₂, 20% glycerol, 0.005% BRIJ^® 35 Detergent, pH 7.5. APMA-free.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of APMA-activated enzyme that will hydrolyze 1µmol MCA-Pro-Leu-Lys-Gly-Leu-DPA-Ala-Arg-NH₂ (Cat. No. 03-32-5032) at 37°C, pH 7.5.

Warning

Toxicity: Standard Handling (A)