Application

ELISA (1:500-1:1000)r>Frozen Sections (1:50-1:500)r>Immunoblotting (1:1000-1:10,000)r>Paraffin Sections (1:50-1:500)

General description

This Anti-Lipoic Acid Rabbit pAb is validated for use in ELISA, Frozen Sections, Immunoblotting, Paraffin Sections for the detection of Lipoic Acid.

Recognizes native lipoic acid. Does not recognize lipoic acid modified by 4-HNE (4-hydroxy-2-noneal) and other lipid peroxidation products.

Rabbit polyclonal antibody supplied as diluted serum. Recognizes native lipoic acid and lipoic acid covalently attached to proteins.

Immunogen

lipoic acid conjugated to KLH

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Does not bind lipoic acid modified by 4-HNE (4-hydroxy-2-noneal) and other lipid peroxidation products. Useful for assessing whether protein-bound lipoic acids have undergone certain forms of oxidative modification. The presence of protein-bound lipoic acid can be used as a marker for mitochondria. Variables associated with assay conditions will dictate the optimal working dilution.

Sasaki, M., et al. 2000. J. Autoimmun.15, 51.Heitzer, T., et al. 2001. Free Radic. Biol. Med.31, 53.Patrick. L. 2000. Altern. Med. Rev.5, 290.Shi, S.S., et al. 1999. Antioxid. Redox Signal1, 123.Humphries, K.M. and Szeda, L.I. 1998. Biochemistry37, 15835.

Packaging

100 µL in Plastic ampoule

Physical form

serum diluted in buffer, 50% glycerol, pH 7.5.

Warning

Toxicity: Standard Handling (A)