Application

ELISA (see comments and application references)

General description

Purified mouse monoclonal antibody. Recognizes human IgG₁ Fc fragment.

This Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069) is validated for use in ELISA for the detection of Human IgG₁, Fc Fragment Specific.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.Jefferis, R., et al. 1986. Immunol. Letters10, 223.

Can be used as a capture antibody for ELISA at 10 µg/ml in PBS without carrier protein. Antibody should be titrated for optimal results in individual systems.r>r>Cross-reactivity by ELISA against human myeloma proteins:r>Human IgG₁: 100%r>Human IgG₂: 0.1%r>Human IgG₃: 0.1%r>Human IgG₄: ﹤0.02%r>Human IgA: ﹤0.1%r>Human IgE: ﹤0.1%r>Human IgM: ﹤0.1%r>r>Cross-reactivity by ELISA against chimeric antibodies:r>Human IgG₁: ﹤0.01%r>Human IgG₂: ﹤0.01%r>Human IgG₃: ﹤0.01%r>Human IgG₄: ﹤0.01%r>Human IgA: ﹤0.01%r>Human IgE: ﹤0.001%r>Human IgM: ﹤0.01%

Packaging

400 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, pH 7.4.

Reconstitution

Following initial use aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)