General description

Note: 1 KU = 1000 units.

Recombinant, Aspergillus niger glucose oxidase. Catalyzes the oxidation of β-D-glucose to D-glucono-Ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-Ω-lactone to gluconic acid. Each molecule of enzyme contains 2 molecules of FAD, which are essential for enzyme activity. Optimal pH = 6.5 with >95% maximal activity at pH 6.0-7.0. Useful for enzymatic assay of glucose. May also be conjugated to antibodies or antigens for use in ELISA procedures.

Recombinant, Aspergillus niger glucose oxidase. Catalyzes the oxidation of β-D-glucose to D-glucono-Ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-Ω-lactone to gluconic acid. Useful for enzymatic assay of glucose concentration.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Heinz, F. and Beushausen, T.W. 1981. J. Clin. Chem. Clin. Biochem. 19, 977.

Packaging

10 ku in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C.

Unit Definition

One unit is defined as the amount of enzyme that will oxidize 1.0 mmol of β-D-glucose per minute at 25°C, pH 7.0.

Warning

Toxicity: Standard Handling (A)