Analysis Note

Major band by SDS-PAGE

General description

Elastase, Human Neutrophil, CAS 9004-06-2, is a serine protease that degrades elastin, collagen, and proteoglycans

Native elastase from human neutrophil. Degrades collagen, elastin, and proteoglycans. Elastase has been implicated in the development of pulmonary emphysema and rheumatoid arthritis.

Native elastase from human neutrophil. A serine protease that degrades elastin, collagen, and proteoglycans. Its overexpression has been implicated in the development of pulmonary emphysema, rheumatoid arthritis, and cystic fibrosis.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Siedle, B., et al. 2002. Biorg. Med. Chem.10, 2855.Junger, W.G., et al. 1992. Biol. Chem. Hoppe-Seyler 373, 691.Selak, M.A. 1992. Thromb. Haemost.68, 570.Baugh, R.J., and Travis, J. 1976. Biochemistry15, 836.

Packaging

100 µg in Glass bottle

50 µg in Plastic ampoule

Physical form

Salt-free.

Preparation Note

Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Reconstitute in 50 mM sodium acetate, pH 5.5, with 200 mM NaCl or in other aqueous buffers. Material is most active near a neutral pH, but to maintain stability upon reconstitution, use in a slightly acidic buffer (pH 5.5) to avoid autolysis.

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Acidic stock solutions are stable for up to 1 month at 4°C or for up to 1 year at -20°C.

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of MeO-Suc-Ala-Ala-Pro-Val-pNA (catNO=454454">Cat. No. 454454) per min at 25°C, pH 8.0.

Warning

Toxicity: Standard Handling (A)