Cathepsin K, His#Tag�, Human 1PC X 25UG

General description

Cathepsin K is a member of the papain superfamily of cysteine proteinases and plays an important role in osteoclast-mediated bone resorption and collagen degradation. It is primarily expressed by osteoclasts and recent studies also suggest its expression in fibroblasts. Irregularities in cathepsin K activity, either too high or too low, contribute to osteopenic disorders such as osteoporosis and pycnodysostosis. This recombinant enzyme contains a C-terminal His Tag™ sequence.

Cathepsin K is a member of the papain superfamily of cysteine proteinases and plays an important role in osteoclast-mediated bone resorption and collagen degradation. It is primarily expressed by osteoclasts. Recently reported to also be expressed in fibroblasts. Irregularities in activity contribute to osteopenic disorders such as osteoporosis and pycnodysostosis. Contains a C-terminal His Tag™.

Note: 1 mU = 1 milliunit.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Delaisse, J.M., et al. 2003. Microsc. Res. Tech.61, 504.Dodds, R.A. 2003. Cell Biochem. Funct.21, 231.Everts, V., et al. 2003. Calcif. Tissue Int.73, 380.Hou, W.S., et al. 2003. Biol. Chem.384, 891.

Packaging

25 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM NaOAC, pH 5.5. The activation was stopped by addition of 10 mM MMTS, which can be removed under reducing conditions.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 0.4 µmol Z-FR-AMC per min at 37°C, pH 5.5.

Warning

Toxicity: Standard Handling (A)