General description

Native cathepsin B from human liver, purified by affinity chromatography. The most investigated enzyme of all lysosomal cysteine proteases. Upregulation and secretion of cathepsin B have been shown to occur in many types of tumors and to correlate positively with their invasive and metastatic capabilities. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers. Purified from human liver by affinity chromatography. Optimal pH: 3.5-6.0.

Native cathepsin B from human liver, purified by affinity chromatography. The most investigated enzyme of all lysosomal cysteine proteases. Upregulation and secretion of cathepsin B have been shown to occur in many types of tumors and to correlate positively with their invasive and metastatic capabilities. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.

Cathepsin B, Human Liver, CAS 9047-22-7, is a purified native cathepsin B from human liver, purified by affinity chromatography. Upregulated in many types of tumors.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kostoulas, G., et al. 1999. FEBS Lett. 455, 286.Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol.389, 281.Keppler, D., et al. 1994. Biochim. Biophys. Acta.1226, 117.Musil, D., et al. 1991. EMBO J.10, 2321.Sloane B.F. 1990. Semin. Cancer Biol.1, 137.

Packaging

5 u in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.

Preparation Note

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-RR-βNA per min at 40°C, pH 6.0 in the presence of DTT.

Warning

Toxicity: Standard Handling (A)