Analysis Note

Negative ControlNalm-6 cells

Positive ControlHPB-MLT cells

Application

Flow Cytometry (5-10 µg/ml)r>Frozen Sections (see comments)r>Immunoprecipitation (see comments)r>Cell Inhibition Assays (see comments)

General description

Protein A purified mouse monoclonal antibody. Recognizes the ~95 kDa β2 integrin subunit (CD18).

Recognizes the ~95 kDa β2 integrin subunit. Blocks the binding of ICAM-1 and ICAM-3 to LFA-1.

Anti-CD18, mouse monoclonal, clone IB4, recognizes the ~95 kDa β2 integrin subunit. It is validated for FC, IP, IHC on frozen sections, and cell inhibition assays.

Immunogen

Human

human peripheral blood monocytes

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Berman, M.E., et al. 1996. J. Immunol.156, 1515.Kaneko, M., et al. 1995. J. Immunol. 155, 2631.Mazzone, A., and Ricevuti, G. 1995. Haematologica80, 161.Kaneko, M., et al. 1995. J. Immunol.155, 2631.Landis, R.C., et al. 1994. J. Cell Biol.126, 529.Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5699.

Blocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x10⁵ cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Warning

Toxicity: Standard Handling (A)