Application

Immunoblotting (1:500, mouse/rat; 1:1000, human, chemiluminescent)r>Immunocytochemistry (see comments)r>Immunoprecipitation (see comments)r>Immunohistochemistry (see comments)

General description

Protein A purified rabbit polyclonal antibody. Recognizes the ~63 kDa calreticulin protein.

Recognizes the ~63 kDa calreticulin protein in heat-shocked HeLa cells.

Anti-Calreticulin (405-417), rabbit polyclonal, recognizes the ~63 kDa calreticulin in heat-shocked HeLa cells. It is validated for WB, ICC, IHC, and IP.

Immunogen

Human

a synthetic peptide [(C)EEEDVPGQAKDEL] corresponding to amino acids 405-417 of human calreticulin, conjugated to KLH

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Does not recognize calreticulin in Drosophila cells. This antibody has been reported to work for immunocytochemistry, immunohistochemistry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.

Janicki, S., et al. 1997. J. Cell Biol. 139, 485.Wada, I., et al. 1997. EMBO J. 16, 5420. Nauseef, W.F., et al. 1995. J. Biol. Chem.270, 4741.Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.

Packaging

50 µL in Plastic ampoule

Physical form

In PBS, 50% glycerol, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)