Calmodulin, His#Tag� Human, 1PC X 50UG

General description

Full-length, recombinant human calmodulin fused to a His Tag™ sequence at the N-terminus. Contains four functional Ca²⁺-binding sites (aa 20-31; aa 56-67; aa 93-104; aa 129-140) with EF-hands (aa 7-42; aa 43-78; aa 80-115; aa 116-148); and a ubiquitination site at Lys²¹. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Through its interaction with Ca²⁺, calmodulin mediates the control of a large number of enzymes, including protein kinases and phosphatases. It is expected to be phosphorylated at Thr⁴⁴ by CaMK4. In addition, it serves as a substrate for various protein tyrosine kinases.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Chou, J.J., et al. 2001.Nat. Struct. Biol.8, 990.Lennon, G., et al. 1996.Genomics33, 151.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 0.2% Protease Inhibitor Cocktail Set VII (Cat. No. 539138).

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)