Angiostatin K1-3; Human; Recombinant; E. coli

General description

Recombinant, human angiostatin expressed in E. coli as a single, nonglycosylated polypeptide chain containing 259 amino acids. Represents a proteolytic fragment containing the first three kringle structures of plasminogen. Acts as a specific inhibitor of endothelial cell growth and angiogenesis. Displays increased inhibitory activity (ED₅₀=70 nM) relative to kringles 1-4 (ED₅₀=135 nm).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Cao, R., et al. 1999. Proc. Natl. Acad. Sci. USA96, 5728.Cao, E., et al. 1997. J. Biol. Chem.272, 22924.Sim, B.K., et al. 1997. Cancer Res.57, 1329.Wu, Z., et al. 1997. Biochem. Biophys. Res. Commun.236, 651.O′Rieilly, M.S., et al. 1994. Cell79, 315.Peterson, T.E., et al. 1990. J. Biol. Chem.265, 6104.Forsgren, M., et al. 1987. FEBS Lett.213, 254.Malinowski, D.P., et al. 1984. Biochemistry23, 4243.

Packaging

250 µg in Glass bottle

Physical form

Lyophilized from 20 mM Sodium Acetate, 4% Mannitol, pH 5.5.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Warning

Toxicity: Standard Handling (A)