Application

ELISA (0.1-1 µg/ml)r>Immunoblotting (1-10 µg/ml)r>Immunofluorescence (2-20 µg/ml)r>Immunohistochemistry (2-20 µg/ml)

General description

Recognizes both the ~55 kDa non-glycosylated and the ~70 kDa glycosylated, secreted Ang-2 protein in cell culture medium. Does not cross-react with Ang-1.Antibody Target Gene Symbol: ANGPT2 Target Synonym: AGPT2, ANG II, ANG-2, ANGIOPOIETIN, ANGIOPOIETIN 2, Angiotensin 2, Angrp Entrez Gene Name: angiopoietin 2 Hu Entrez ID: 285 Mu Entrez ID: 11601 Rat Entrez ID: 89805

Anti-Angiopoietin-2, rabbit polyclonal, recognizes the ~55 kDa non-glycosylated and the ~70 kDa glycosylated, secreted Ang-2 protein. It is validated for ELISA, WB, IF, and IHC.

Immunoaffinity purified rabbit polyclonal antibody. Recognizes both the ~55 kDa nonglycosylated and the ~70 kDa glycosylated forms of angiopoietin-2.

Immunogen

a synthetic peptide corresponding to a 21 amino acid cytoplasmic sequence at the N-terminus of mouse angiopoietin-2

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

The immunogenic peptide sequence is 71% (15/21 amino acids) conserved in the human angiopoietin-2 protein. Does not cross-react with angiopoietin-1. Variables associated with assay conditions will dictate the optimal working dilution.

Maisonpierre, P.C., et al. 1997. Science 277, 55.Davis, S., et al. 1996. Cell 87, 1161.Suri, S. et al. 1996. Cell 87, 1171.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)