a-ph-Hist H3(S10); 3H10;FITC 100ug

Analysis Note

ControlMitotic HeLa cells (IF).

Application

Research Sub CategoryHistones

Western Blot Analysis: 0.5-5 µg/mL of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog # 17-306) treated with colcemid.

Research CategoryEpigenetics & Nuclear Function

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC Conjugate is a Mouse Monoclonal for detection of Histone H3 phosphorylated at serine 10. This mAb, also known as H3S10p,is labeled with Fluorescein isothiocyanate (FITC), published in peer reviewed journals & validated in WB, ICC & IF.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogen

Epitope: Ser10

A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3. Clone 3H10.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified mouse monoclonal IgG in buffer containing PBS with 0.05% sodium azide, pH7.1.

Protein G Purified

Quality

Immunocytochemistry: Mitotic HeLa cells showed positive chromosome staining with 4µg/mL of this antibody.

Specificity

Broad species cross-reactivity is expected.

Recognizes histone H3 phosphorylated at Ser10, MW 17 kDa.

Storage and Stability

Stable for 1 year at from date of receipt.

Target description

17 kDa