Analysis Note
ControlA431 carcinoma cells.
Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting.
Application
Research CategorySignaling
Research Sub CategoryGeneral Post-translation Modification
Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, recombinant clone 4G10™, biotin conjugate validated for use in WB.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate
Product Description: Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.
Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.
Immunogen
Phosphotyramine-KLH
Legal Information
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Protein G Purified
Biotin-conjugated recombinant mouse IgG2bκ in PBS containing 0.05% sodium azide. Liquid at 2-8°C.
Quality
Routinely evaluated by western blot to detect tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.
Specificity
Tyrosine-phosphorylated proteins from all species.
Storage and Stability
Stable for 9 months at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. DO NOT FREEZE!
Target description
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
This product has met the following criteria to qualify for the following awards: