ANTI-#-ACTIN MOUSE MAB (1A4) 1PC X 500UL

Analysis Note

Negative ControlFibroblasts, striated muscle, or myocardium

Positive ControlSmooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary

Application

Frozen Sections (1:50-1:75)r>Immunoblotting (see comments)r>Paraffin Sections (1:50-1:75)

General description

Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.

This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin.

Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Antibody Target Gene Symbol: ACTA2 Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN Entrez Gene Name: actin, alpha 2, smooth muscle, aorta Hu Entrez ID: 59 (Related Antibodies: 113200) Rat Entrez ID: 81633

Immunogen

a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.

Human Smooth Muscle

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Rannov-Jennsen, L., et al. 1990. Lab Invest.63, 532.Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.

Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

500 µL in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.

Warning

Toxicity: Standard Handling (A)