Anti-Lin28

Analysis Note

ControlNT2/D1 cell lyate

Application

Anti-Lin28 Antibody is a Rabbit Polyclonal Antibody for detection of Lin28 also known as Protein lin-28 homolog A & has been validated in WB.

General description

Lin28 is a developmentally regulated RNA binding protein (RBP). Its down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells. Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancer. The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance. Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.

Immunogen

Histidine-tagged recombinant protein corresponding to human Lin28.

Quality

Evaluated by Western Blot in NT2/D1 cell lyate.

Western Blot Analysis: 1:5,000 dilution of this antibody detected Lin28 in 10 µg of NT2/D1 cell lysate.

Specificity

Other homologies: Rat and Mouse 98% and 97% sequence homology

Target description

~28 kDa observed