Application

Research Sub CategoryHistones

Research CategoryEpigenetics & Nuclear Function

This Anti-monomethyl Histone H4 (Lys20) Antibody, Alexa Fluor^® 647 Conjugate is validated for use in ICC for the detection of monomethyl Histone H4 (Lys20).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The histones have an amino terminal tail, a globular domain, and a carboxy-terminal tail. The four core histones, H2A, H2B, H3 and H4 assemble into the octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped in a sequence-independent manner around the octamer, forming the basic subunit of chromatin, the nucleosome. Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. This product is a conjugated version of Cat. No. 07-748.

Immunogen

Linear peptide corresponding to Histone H4 monomethylated at Lys20.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Physical form

Protein A purified

Purified rabbit conjugated antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% sodium azide.

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected monomethyl Histone H4 (Lys20) in HeLa cells.

Alexa Fluor^® is a registered trademark of Life Technologies™.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~10 kDa observed (Refer to Cat. No. 07-748)