Analysis Note

ControlHeLa nuclear extract.

Application

Research Sub CategoryTranscription Factors

Use Anti-Sp1 Antibody (Rabbit Polyclonal Antibody) validated in ChIP, EMSA, IF, IP, WB to detect Sp1 also known as Sp1 transcription factor, specificity protein 1.

Research CategoryEpigenetics & Nuclear Function

Immunoprecipitation:
4 µg of this lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract.
NOTE: A previous lot successfully immunoprecipitated the α-1 subunit using the Catch and Release v2.0 Reversible Immunoprecipitation System (Catalog # 17-500). See manual for more information and protocol: www.millipore.com

Chromatin Immunoprecipitation:
Reported by an independent laboratory.

Immunofluorescence:
Reported by an independent laboratory.

Electrophoretic Mobility Shift Assay (EMSA):
1 µL of a previous lot of this antibody has been reported by an independent laboratory to supershift DNA complexes containing recombinant Sp1 and endogenous Sp1 from HeLa cells in EMSA.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Sp1 is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 95 kDA. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Sp1 has always been described as a ubiquitous transcription factor that is required for the constitutive and inducible expression of a variety of genes, such as in cell cycle or mammalian development.

Immunogen

Full length human Sp1 protein.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: MABE550

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Format: Purified

Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.

Quality

Routinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis:
1:500 dilution of this lot detected SP1 on 10 µg of HeLa lysates.

Specificity

Expected to cross-react with rat.

Recognizes Sp1, Mr 105 kDa and 95 kDa.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

Varies