Analysis Note

ControlBrain tissue.

Application

Detect NR2A using this Anti-NR2A Antibody validated for use in IP & WB.

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & Receptors

Immunoprecipitation:
4 µg of a previous lot immuno-precipitated NR2A from rat brain hippocampus membranes and rat brain microsomal preparations.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

NR2 or glutamate receptor, ionotropic, N-methyl D-aspartate 2 (NMDA2; NMDAR2) along with the NR1 subunit forms a heterodimer that make up the NMDA receptor. By alternative splicing there are various isoforms of NR2 classified as NR2A through D. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. The NR1 subunit binds glycine while the NR2 subunit binds glutamine for activation. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.

Immunogen

6His-tagged fusion protein corresponding to amino acids 1265-1464 of mouse NMDA receptor 2A (NR2A).

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified rabbit polyclonal IgG in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.

Format: Purified

Quality

Evaluated by western blot in rat brain microsomal preparations (Catalog # 12-144).

Western Blot Analysis:
0.5-2 µg/mL of this antibody detected NR2A in rat brain microsomal preparations (Catalog # 12-144).

Specificity

Recognizes NR2A, MW ~170 kDa.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Target description

~170 kDa