Application

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Histone H2A.X in human pancreas and human colon tissue sections.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Histone H2A.X in H2A and H2A.X recombinant protein.

Anti-Histone H2A.X Antibody, Cat. No. 07-627, is a highly specific rabbit polyclonal antibody that targets Histone H2A.X and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

General description

Histone H2AX (UniProt: P16104; also known as H2a/x, Histone H2A.X) is encoded by the H2AFX (also known as H2AX) gene (Gene ID: 3014) in human. Histones are highly conserved basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries that require DNA as a template. The histone H2A.X is a variant member of the H2A family of histones that is distinguished from other H2A histones by a unique carboxy-terminal sequence. This C-terminal tail becomes phosphorylated following double-stranded DNA breaks following irradiation or apoptosis. This unique sequence is highly conserved throughout eukaryotic evolution and is rapidly phosphorylated by ATM or ATR in response to DNA double-strand breaks. H2A.X phosphorylation is important in the formation of a stable repair complex at the site of DNA damage.

Immunogen

KLH-conjugated, synthetic peptide (CSATVGPKAPSGGKKA) corresponding to amino acids 121-135 of human histone H2A.X, with an N-terminal cysteine added for conjugation purposes. The immunizing sequence has 13/15 identical amino acids in mouse.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Rabbit antiserum containing 0.05% sodium azide and 30% glycerol. Liquid at -20°C.

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Histone H2A.X in Jurkat cell lysate.

Specificity

This rabbit polyclonal antibody detects Histone H2AX in human cells. It targets an epitope with in 15 amino acids from the C-terminal region.

Target description

~15 kDa; 15.15 kDa calculated.