Analysis Note

ControlHeLa cell lysate, HL-60 cell lysate

Application

Anti-Ago 2 Antibody is a Rabbit Polyclonal Antibody for detection of Ago 2 also known as argonaute 2 & has been tested in WB.

General description

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.

Provides endonuclease activity to RNA-induced silencing complexes (RISC). Cleaves siRNA/mRNA heteroduplexes bound to RISC. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).

Immunogen

GST fusion protein corresponding to residues 7-48 of human argonaute 2 (Ago2).

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Quality

Routinely evaluated by immunoblot in RIPA and cytosolic lysates from HeLa cells.

Specificity

Recognizes Ago 2, also known as eukaryotic translation initiation factor 2C, 2 (EIF2C 2), MGC3183 and Q10.

Target description

100 kDa