Analysis Note

ControlPositive Control Included: Non-Stim A431 Cell Lysate (12-301)

Application

Research CategoryEpigenetics & Nuclear Function

Detect CoREST with Anti-CoREST Antibody (Rabbit Polyclonal Antibody), that has been shown to work in ChIP, ICC, IHC, IP & WB.

Research Sub CategoryChromatin Biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The CoREST gene encodes a functional corepressor required for regulation of neural specific gene expression. REST (RE1 silencing transcription factor 1) can mediate extraneuronal restriction by imposing either active repression via histone deacetylase recruitment or long term gene silencing using a distinct functional complex. Silencing of neuronal specific genes requires the recruitment of an associated corepressor, CoREST, that serves as a functional molecular beacon for the recruitment of molecular machinery that imposes silencing across a chromosomal interval, including transcriptional units that do not themselves contain REST response elements.

Immunogen

GST fusion protein corresponding to residues 109-293 of human CoREST.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein A purified

Protein A Purified immunoglobulin in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, and 0.05% sodium azide as a preservative.

Format: Purified

Quality

routinely evaluated by immunoblot in RIPA lysates from A431, 3T3/A31, Jurkat,and Hela cells

Specificity

Recognizes CoREST.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

66 kDa