Analysis Note

ControlHeLa whole cell lysate, U2OS cell lysate

Application

Use Anti-dimethyl-Histone H3 (Lys27) Antibody (rabbit polyclonal antibody) validated in Mplex, PIA, WB, ChIP-seq, DB to detect dimethyl-Histone H3 (Lys27) also known as H3K27me2, Histone H3 (di methyl K27).

Research Sub CategoryHistones

Research CategoryEpigenetics & Nuclear Function

Additional Referenced Research Applications:
Dot Blot, Immunocytochemistry (cells)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Anti-dimethyl-Histone H3 (Lys27)

Immunogen

KLH-conjugated, synthetic 2X-branched peptide containing the sequence ...AR_me2KSA... in which _me2K corresponds to dimethyl-lysine at residue 27 of human histone H3

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified IgG in 0.1M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% NaN3.

Peptide affinity purified

Quality

Routinely evaluated by immunoblot on acid extracted proteins from HeLa cells.

Specificity

Broad species cross-reactivity expected, based on sequence homology

Recognizes dimethylated histone H3 (Lys27).

Storage and Stability

Stable for 2 years at 2-8°C from date of shipment.

Target description

17 kDa