Application

Research Sub CategoryNuclear Receptors

This Anti-trimethyl Histone H3 (Lys27) is validated for use in Immunocytochemistry for the detection of trimethyl Histone H3 (Lys27).

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2.

Immunogen

Branch peptide corresponding to trimethyl-Histone H3 (Lys27).

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein A purified

Purified rabbit IgG in PBS containing 15 mg/mL BSA and 0.1% Sodium Azide.

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl-Histone H3 (Lys27) in HeLa cells.
Alexa Fluor^® is a registered trademark of Life Technologies™.

Specificity

The unconjugated parent antibody of this antibody is Cat. No. 07-449.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~17 kDa observed. The unconjugated parent antibody (Cat. No. 07-449) has an observed molecular weight at ~17 kDa